Journal: PLoS ONE

Article Title: Massively Parallel Interrogation of Aptamer Sequence, Structure and Function

doi: 10.1371/journal.pone.0002720

Figure Lengend Snippet: (A) Representative images of an aptamer microarray incubated with labeled IgE. The eight identical subarrays were individually yet simultaneously interrogated with decreasing concentrations of IgE (100 nM to 0.1 nM). Insets (30 nM IgE) demonstrate discrete features of uniform size and intensity. (B,C) Subarrays incubated with 10 nM IgE from two separate experiments were used to analyze intra- and inter-slide data correlation (B and C, respectively). Both correlation analyses demonstrated a high degree of reproducibility, with r 2 values greater than 0.990. The slope of the correlation trend is indicative of relative fluorescence intensities. Slide 2 was washed less stringently than slide 1, hence the higher fluorescence intensities of its features are reflected in a slope of 1.84 (vs. 1.04 in the intraslide comparison where all wash conditions were constant). Averaging of triplicate data points (c, red circles) effectively eliminated outliers seen in the plot of individual data points (black circles). Average percent coefficient of variability (%CV) values for intra- and interslide subarray comparisons were determined to be 5.76% and 6.86%, respectively (see ).

Article Snippet: Indeed, the Combimatrix electrode array microarray format has the potential to be used much like the Agilent arrays were used here but with an electronic readout.

Techniques: Microarray, Incubation, Labeling, Fluorescence